Quick blunting kit neb e1201s
WebQuick Blunting™ Kit E1201S 20 reactions Exp: 2/15Lot: 0071302 RECOMBINANT Store at –20°C Description: The Quick Blunting Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for ef-ficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase (NEB WebE1201S Quick Blunting Kit Packaging Unit: 20 reactions ... € 88,00 -+ Add to Cart E1201L Quick Blunting Kit Packaging Unit: 100 reactions Code NACRES: NA.54 € 355,00 -+ Add to …
Quick blunting kit neb e1201s
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WebApr 7, 2015 · Protocol for the Quick Blunting Kit (E1201) 1. Mix the following components in a sterile microfuge tube: AMOUNT Purified DNA (up to 5 μg) 1–19 μl 10X Blunting... 2. … WebThe Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning …
WebBlunting. Blunting is a process by which the single-stranded overhang created by a restriction digest is either "filled in", by the addition of nucleotides on the complementary strand using the overhang as a template for polymerization, or by "chewing back" the overhang, using an exonuclease activity. Vectors and inserts are often "blunted" to ... WebThe Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature . (25°C ) Ligase Quality. Ligation Time Course: LITMUS 28i vector was cut with either EcoRV …
WebThe Quick Blunting Kit is used to convert DNA with incompatible 5´ or 3´ overhangs to 5´ phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. … WebProduct Name: Quick Blunting™ Kit Catalog #: E1201S/L Kit Components: Blunting Enzyme Mix (M1201) 10X Blunting Buffer (B1201) Deoxynucleotide Solution Mix (1 mM) (N1201) ... www.neb.com [email protected] New England Biolabs Product Specification. NEW ENGLAND Inc. nqa ISO 9001 Registered Quality Management nqa ISO 14001 Registered
WebProduct Name: Quick Blunting™ Kit Catalog Number: E1201S Packaging Lot Number: 10080108 Expiration Date: 08/2024 Storage Temperature: -20°C Specification Version: PS-E1201S/L v1.0 Quick Blunting™ Kit Component List NEB Part Number Component Description Lot Number Individual QC Result N1201AVIAL dNTP Mix 10080111 Pass
WebProduct Name: Quick Blunting™ Kit Catalog Number: E1201S Packaging Lot Number: 10180930 Expiration Date: 08/2024 Storage Temperature: -20°C Specification Version: PS-E1201S/L v1.0 Quick Blunting™ Kit Component List NEB Part Number Component Description Lot Number Individual QC Result N1201AVIAL dNTP Mix 10160894 Pass shopkins milly mopsWebThe Quick Blunting Kit is used to convert DNA with incompatible 5´ or 3´ overhangs to 5´ phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA Polymerase (NEB #M0203) which has both 3´ → 5´ exonuclease activity and 5´ → 3´ polymerase activity. T4 Polynucleotide Kinase ... shopkins milk cartonWebProduct Class: Kit Quick Blunting™ and Quick Ligation™ Kits The large size of this product was discontinued on 12/31/2024. The small size will continue to be available. Product Introduction NEB offers the Quick... shopkins micro martshopkins milk cerealWebE1201S. 20 REACTIONS. £85.00. The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt … shopkins mouthwash characterWebManufacturer: New England Biolabs, Inc. E1201S. The Quick Blunting Kit is used to convert DNA with incompatible 5' or 3' overhangs to 5' phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA Polymerase which has both 3' to 5' exonuclease activity and 5' to 3 polymerase activity. shopkins movies on youtubeWebQuick Blunting Kit, Article Number: E1201S Sign In (0) Products & Applications. back Cloning / Synthetic ... Quick Blunting Kit Packaging Unit: 20 reactions € 88,00 -+ Add ... shopkins minty paws