Peasy t1

Author: hvvn

August undefined, 2024

WebpEASY-T1 Simple (linearized) TA cloning vector suitable for blue-white selection, with kanamycin and ampicillin resistance markers and a T7 promoter but no multiple cloning … WebpEASY -T1 Cloning Kit is designed for cloning and sequencing Taq-amplified PCR products (3′-A overhangs). The vector is suitable with T7 RNA polymerase-mediated in vitro RNA …

pEASY®-T1 Cloning Kit

WebDec 6, 2024 · The tmexCD1.2-toprJ1 and tmexCD2.2-toprJ2 gene clusters were amplified by PCR and ligated to the pEasy-T1 vector (Transgen, Beijing, China) using Gibson cloning (Gibson Assembly Cloning Kit, NEB). The primers used for subcloning are listed in Table S1 (available as Supplementary data at JAC Online). WebApr 24, 2015 · The reference plasmid pEASY-T1-STY2879 was constructed as follows: the full length STY2879 gene, which was from the S. Typhi strain CT18, was amplified by a set of primers, STY2879-F: 5’-3’ ttacaacttaaagagtttcgc and STY2879-R: 5’-3’ atgacaatagaagttcaaagg, and the PCR products (774 bp) were ligated into the pEASY-T1 … homemade strawberry juice recipe

pEASY®-T1 Simple Cloning Kit - CT111 Civic Bioscience

WebpEASY® -T1 Cloning Kit Catalog Number：CT101-01 Price：$89.02 Specification： 20 rxns 60 rxns Quantity： Manual Vector Information MSDS COA Inquire Now Buy Now Add to … WebContext 1 ... determine the detection limit, PCR and LAMP were performed with tenfold serial dilutions of the plasmid pEASY ® -T1-B637 as template. PCR products were detected by agarose gel elec-... WebOct 15, 2024 · To compare the detection sensitivities between dLAMP and qPCR, we gradient diluted pEASY-T1-mpb70 plasmid and added them to dLAMP and qPCR systems, respectively. The qPCR detection resulted in good linearity between input templates and measured concentration (R 2 = 0.9977; Fig. 3); a wide range of templates can be … hindustan pressing private limited

High-performance detection of - ScienceDirect

Characterization and expression of glucosamine-6-phosphate

Webpeasy 系列克隆载体; 相关产品; 克隆感受态细胞; 点突变; 核酸纯化 . 基因组dna纯化; 质粒dna纯化; dna产物纯化; rna纯化; 相关产品; 二代测序 . dna建库; rna建库; 相关产品; mngs; … WebApr 6, 2024 · All PCR products were separated on a 1.2% agarose gel, and the target bands were purified and then ligated into the pEASY-T1 vector (TransGen, Beijing, China). The PCR products were sequenced by Sangon Biotech (Shanghai, China) [ 25 ]. hindustan petroleum dividend history hindustan newsprint ltd

"WebThis fragment contains 285 bp upstream of the Hsp-3start codon and was ligated into the T1 vector using the pEASY-T1 Cloning kit (Transgen) (Figure 1, step 2) to generate sufficient material for subsequent manipu- lations. " - Peasy t1

Peasy t1

Single nucleotide polymorphism at alcohol dehydrogenase-1B is ...

WebPeasy T1 Simple Cloning Vector System, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … WebPeasy T1 Simple Cloning Vector, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, …

Did you know?

WebMar 2, 2024 · The amplified GhGT23 fragment was subcloned into pEASY-T1 (TransGen Biotech, Beijing, China), thereby creating pEASY-T1::GhGT23. Flanking BamHI and SalI restriction sites were then used to cut GhGT23 from pEASY-T1::GhGT23, followed by ligation into the pBI21, pBin438, pGAL4 DBD, and pGEX6p-1 expression vectors. WebM-Wave Drag-T1 M-Wave Drag-T2 M-Wave Peasy; Zum Angebot * x Wir verlinken auf ausgewählte Online-Shops und Partner, von denen wir ggf. eine Vergütung erhalten. Zwischenzeitliche Änderung der Preise, Lieferzeit und -kosten möglich. Preise inkl. MwSt, ggf. zzgl. Versand. Wenn Sie eine ältere Version des Vergleichs aufrufen oder ansehen ...

WebJan 30, 2015 · The amplified DNA was purified using an AxyPrep DNA gel extraction kit (Axygen), inserted into the pEASY -T1 Cloning Vector (TransGen, Beijing, China) and transformed into E. coli Trans 1-T1 competent cells (TransGen, Beijing, China). Positive clones were analyzed by PCR using vector-specific primers. WebpEASY® -T1 Simple Cloning Kit Catalog Number：CT111-01 Price：$97.24 Specification： 20 rxns 60 rxns Quantity： Manual Vector Information MSDS COA Inquire Now Buy Now …

WebJun 15, 2014 · The PCR product was purified and cloned into pEASY-T1 vector and transformed into E. coli DH5α. Positive clones were identified and verified by sequencing pEASY-T1-GFA1 with M13 primers (Transgen Biotech, Beijing, China) and comparison with the GFA1 sequence database. WebConstruction of Clone Vectors pEASY-T1-2f PF4 and pEASY-T1-3f PF4. Source publication Polycistronic Expression of Human Platelet Factor 4 with Heparin-Neutralizing Activity in …

WebpEASY ® - T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY ®-T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products. •5 …

WebpEASY® -T1 Cloning Kit 目录号： CT101-01 单价：￥620 规格： 20 rxns 60 rxns 数量： - + 说明书载体信息质检报告立即下单加入购物车产品详情介绍 pEASY®-T1 Cloning … hindustan news paper hajipur vaishaliWebpEASY-T3 (linearized) TA cloning vector that allows the cloned PCR product to be excised with EcoRI or NotI. Sequence Author: TransBionovo (TransGen Biotech) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7kPlasmids:Basic Cloning Vectors More Plasmid Sets No matches hindustan news paper hindiWebBraccialini - Braccialini - Accessoires textile - Accessoires sacs - Parapluies - Rose 60,00 € - Spartoo hindustan petroleum corporation limited hqWebpEASY® – T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY®- T1 Cloning vector. It is designed for cloning and sequencing Taq -amplified PCR products. … homemade strawberry sherbet recipeWebpEASY®- T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY®-T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products.•5 … hindustan phosphates pvt. ltdWebJan 31, 2014 · The EcoR I/Not I digested fragments of pEASY-T1-ADH1B were ligated with pPICZB, resulting in pPICZB-ADH1B. The recombined plasmid was transfected by electroporation of Pichia pastoris. Enzyme purification and assays. Cells were harvested by centrifugation and washed with 50 mM Tris–HCl, pH8.0 (buffer A). The cell paste was … hindustan pressing pvt ltdWebpEASY® -Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa. • 5 minutes fast ligation of Pfu-amplified PCR products. hindustan newspaper job search