WebJun 22, 2016 · PubMed Abstract: The multidomain CCCTC-binding factor (CTCF), containing a tandem array of 11 zinc fingers (ZFs), modulates the three-dimensional organization of chromatin. We crystallized the human CTCF DNA-binding domain in complex with a known CTCF-binding site. While ZF2 does not make sequence-specific … WebGeneral description. CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator ...
NPM1c impedes CTCF functions through cytoplasmic ... - Nature
WebMar 3, 2024 · The shorter CTCF forms are expected to lack several domains; in the ~ 120 kDa form, we estimate that most of the N-terminal part that is responsible for cohesin retention and formation of genomic loops (Pugacheva et al. 2024) is missing, but all eleven zinc-finger still exist. WebChIP results obtained with the Diagenode antibody directed against CTCF. ChIP was performed with the Diagenode antibody against CTCF (cat. No. C15410210) on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. church suwanee ga
Anti-BPTF (VCU19) from rabbit, purified by affinity …
WebAug 17, 2024 · CTCF is required for peripheral nerve ensheathment a Excised exon 8 of the floxed Ctcf allele by Dhh-Cre. b Co-labeling of CTCF with SOX10 in control and mutant sciatic nerves at P7 (n = 3 animals ... WebJun 8, 2004 · The protein CTCF plays an essential role in the action of a widely distributed class of vertebrate enhancer-blocking insulators, of which the first example was found in a DNA sequence element, HS4, at the 5' end of the chicken beta-globin locus. ... a 38-kDa nucleolar phosphoprotein that is concentrated in nuclear matrix preparations. In this ... WebThis 70 kDa band for CTCF has been described previously [197,245] as a possible N-terminally truncated CTCF protein generated by alternative translation of CTCF mRNA which is known to enhance CTCF ... church swag box