WebNov 25, 2024 · The TEM analysis, performed here revealed the Cu-Amp-CPG nanocatalyst (Fig. 4) displayed a well-dispersed and uniform pattern of nanoparticles in the size … WebJul 1, 2024 · CpG nanoparticles (CpG NPs) were fabricated with CpG and PEI under the crosslinking of genipin, just as ovalbumin nanoparticles [ 23 ]. In brief, CpG (500 μg) was dissolved in 700 μL of genipin solution (0.3% w/v) to stir 24 h. Then, 350 μL PEI solution (Mw = 10 kDa, 1.5 mg/mL) was added gradually under stirring for 24 h.

Optimization, Production, and Characterization of a CpG …

WebNov 1, 2024 · CpG oligodeoxynucleotides (CpGs) can induce an anti-tumor immune response, but also uniquely cause direct lymphoma cytotoxicity. To improve the delivery and efficacy of CpGs, we utilized a tri-ethylene modified CpG conjugated gold nanoparticle (tmCpG NP) platform that is compatible with both class B and class C CpGs, to treat … Web1 day ago · The most successful medical intervention for preventing infectious diseases is still vaccination. This effective strategy has resulted in decreased mortality and extended life expectancy. However, there is still a critical need for novel vaccination strategies and vaccines. Antigen cargo delivery by nanoparticle-based carriers could promote superior … john wayne airport to honolulu

Development of a broadly active influenza intranasal vaccine

WebMar 14, 2024 · This novel nanoparticle (NP) adjuvant was co-formulated with a computationally optimized broadly reactive antigen (COBRA) for hemagglutinin (HA), which is broadly reactive against influenza strains. M7 was combined at different ratios with CpG and tested for in vitro immune responses and cytotoxicity. WebMar 24, 2024 · This novel nanoparticle (NP) adjuvant was co-formulated with a computationally optimized broadly reactive antigen (COBRA) for hemagglutinin (HA), which is broadly reactive against influenza strains. M7 was combined at different ratios with CpG and tested for in vitro immune responses and cytotoxicity. WebCpG-B and CpG-C conjugation to NPs enhances both local and systemic CD8 + T-cell memory recall. (A) Immunization schedule; C57BL/6J mice were immunized with 4 µg CpG-B and 10 µg OVA conjugated to NPs on days 0 and 14. Two months later, memory was recalled with 4 µg of the different CpG formulations and 10 µg NP-conjugated OVA. how to hack win